New IMMULITE® Free T3

DPC has recently released IMMULITE Free T3 for the measurement of free T3 in serum. Measure-ments of T3, either total or free, are used primarily to confirm the diagnosis of hyperthyroidism, especially in cases of T3 thyrotoxicosis and in hyperthyroidism associated with low TSH and normal T4 levels.

IMMULITE Free T3 is a solid-phase, competitive, sequential, chemiluminescent enzyme immuno-assay. The assay uses an analog procedure in which T3 with a biotinylated amino group serves as the T3 analog tracer. The biotin of the analog blocks its binding to thyroid hormone binding globulin (TBG) and transthyretin; a combination of chemical blocking agents prevents binding to albumin. Thus, the endogenous proteins are effectively blocked from binding the T3 analog tracer, to ensure an accurate estimate of free T3.

The IMMULITE Free T3 kit requires a total incubation time of 60 minutes and has a calibration range of 1 to 40 pg/mL. It is available in kit sizes of 100 and 500 tests.