Kursus: Immunoloogilised meetodid

Tallinn
8.-9.aprill 2003.a.
Hotel PIRITA
Regati Boulevard 1, Tallinn,
conference room "HIIU"

Päevakava

Pidid kursuselt

A Course "Immunological Methods" was held on 8-9.04.2003 in Tallinn, Estonia.

Lecturers: Ingrid Ferdinandsson (DPC Scandinavia Technical Services Manager) Nicholas Holthuis (MD, Director of Blood Centre Skåne, Lund University Hospital)

Participants: all together - 44 participants from 5 countries (including lecturers and DPC employees): Sweden, Finland, Latvia, Lithuania, Estonia

This course was organized for lab.doctors, lab.technicians, chemists. First day (8.04) was about basics aspects about immunoassays in general. Mr.Nicholas Holthuis (from Lund University Hospital, Sweden) gave lectures about principles of immunochemistry. Groups of immunoassays (competitive, immunometric), antigen-antibody reaction, immunoassay reagents (binding proteins, labels, buffers, separation systems), optimisation and validation methods, uncertainty measurements were described.

Lectures on second day (9.04) were made by Ingrid Ferdinandsson. First talk was about expectations for immunoassays - review of basic concepts, what to expect for assay precision, and planning the goals (what is the goal, what is significant change, how many levels to use, what to do, if controls are out of range), and some examples about precision profile, analytical sensitivity, functional sensitivity. Next talks were about interferences of immunoassays, preanalytical variabilities, plasma vs serum as sample type, matrix effect, heterophilic antibodies' interference, mechanical inteference, meaning of "hook effect". Separate topic was about external controls - examples from different Immulite&Immulite2000 assays (insulin, CEA, cortisol etc) in CAP and NEQAS schemes.

Last presentation of the course was about light immunoassay technology (with animations, what made underestanding of the technology better and easy-to-remember).